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CABRI:DSMZ_MUTZ
Accession_number ACC 219
Cell_line_name 8505C
Brief_description confirmed as human with IEF of AST, LDH, NP established from the primary tumor of a 78-year-old woman with thyroid carcinoma (undifferentiated carcinoma, histologically a largely papillary adenocarcinoma with some spindle, polygonal and giant cells)
Description human thyroid carcinoma established from the primary tumor of a 78-year-old woman with thyroid carcinoma (undifferentiated carcinoma, histologically a largely papillary adenocarcinoma with some spindle, polygonal and giant cells) confirmed as human with IEF of AST, LDH, NP
Depositor Dr. M. Akiyama, Radiation Effects Research Foundation, Hiroshima, Japan
Morphology epithelial-like adherent cells growing as monolayer
Culture_conditions 90% RPMI 1640 + 10% FBS split confluent culture 1:5 every 2-3 days or 1:10 every 4-5 days using trypsin/EDTA at 37C with 5% CO2 cell harvest of about 20 x 106 cells/175 cm 2
Viruses ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -, HTLV-I/II -, SMRV -
Properties cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, GFAP -, HMB-45 -, neurofilament -, thyroglobulin -, vimentin +
Release_conditions -
Hazard -
Storage frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule
Doubling_time about 30-40 hours
Mycoplasma negative in DAPI, microbiological culture, RNA hybridization assays
Fingerprint multiplex PCR of minisatellite markers revealed a unique DNA profile
Cytogenetics human hypotriploid karyotype with 16% polyploidy - 65(57-68)<3n>XX, -X, -2, +3, -7, -10, +11, -13, -16, -18, -19, +2mar, del(1)(q13), del(3)(q22), ?del(4)(q21), i(8q)
Bibliographic_reference Ito et al., Int J Oncol, 4: 49 (1994) [PMID: ]
SRS Release 7.1.3    Copyright © 1997-2003 LION bioscience AG. All Rights Reserved. Terms of Use Feedback